Restriction-site PCR: a direct method of unknown sequence retrieval adjacent to a known locus by using universal primers.
نویسندگان
چکیده
منابع مشابه
Rapid acquisition of unknown DNA sequence adjacent to a known segment by multiplex restriction site PCR.
The determination of unknown DNA sequences around a known locus has important applications in molecular genetics, specifically in genomic walking and genome mapping. Several PCR-based methods have been reported to address this issue, but they often involve multiple, time-consuming steps. We have previously described a technique known as restriction site PCR (RS-PCR) that allows sequence acquisi...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1993
ISSN: 1088-9051
DOI: 10.1101/gr.2.4.318